By clinical oral examination, some of the physical and biochemical analysis of human unstimulated whole saliva have been studied with their effects on the retention of acrylic denture base. The following parameters were studied: flow rate, pH, total protein, density, surface tension, viscosity and film thickness. Total protein concentration was found to be responsible for physical and chemical changes specially viscosity. There was a direct positive correlation between total protein and surface tension, viscosity, density and film thickness, while there was a negative correlation between total protein and with flow rate and pH. The results indicated that in both the first and the third groups, the physical and mathematic analysis for retention showed non significant changes while in the second group showed significant changes. The results in all groups showed that there was a positive correlation between force and wetted area and this force was due to wettability...

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