The pharmacological studies, acute toxicity, sub acute or repeated dose 28 day oral toxicity study and sub chronic or repeated dose 90 day oral toxicity study of HF cross bred cow urine as well as routine comparative study of urine from different breeds of cows were carried out in the present investigation in wistar albino rats.In conclusion, crossbred milking HF cows’ urine and indigenous cow breeds showed hepatoprotective effect and wound healing properties. Crossbred milking HF cows’ urine was found to be safe at the used dosage in terms of various hematological and biochemical parameters.

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