The ultimate goal in the treatment of destructive periodontal disease is the regeneration of the lost attachment apparatus i.e. the formation of new cementum, a new periodontal ligament and new alveolar bone. Tetracycline hydrochloride as a root conditioner was evaluated in vivo studies. Various techniques for application of tetracycline hydrochloride were experimented. Burnishing technique was found to be the best technique among them. It was found that there was lesser tissue destruction and better regeneration of periodontal tissues if tetracycline hydrochloride is used as a root conditioner during periodontal regeneration procedures.

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