High Quality Content by WIKIPEDIA articles! Reverse phase protein microarray (RPMA) is a protein array designed as a micro- or nano-scaled dot-blot platform dot-blot that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available. Technically, minuscule amounts of a) cellular lysates, from intact cells or laser capture microdissected cells, b)body fluids such as serum, CSF, urine, vitreous, saliva, etc., are immobilized on individual spots on a microarray that is then incubated with a single specific antibody to detect expression of the target protein across many samples. One microarray, depending on the design, can accommodate hundreds to thousands of samples that are printed in a series of replicates. Detection is performed using either a primary or a secondary labeled antibody by chemiluminescent, fluorescent or colorimetric assays. The array is then imaged and the...

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