Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Random Coil Index (RCI) predicts protein flexibility by calculating inverse weighted average of backbone secondary chemical shifts and predicting values of model-free order parameters as well as per-residue RMSF of NMR and molecular dynamics ensembles from this parameter. The key advantages of this protocol over existing methods of studying protein flexibility are (i) it does not require prior knowledge of a protein's tertiary structure, (ii) it is not sensitive to the protein's overall tumbling and (iii) it does not require additional NMR measurements beyond the standard experiments for backbone assignments.

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